I enjoy playing my bass guitar with Rocksmith. However I do not use Windows at all and wanted it to work with Kali Linux 2021.2, a Debian 10 Distro.
This is how I got it to work with my audio and Nvidia GeForce RTX 2070
Install crossover bin file
wget https://media.codeweavers.com/pub/crossover/cxlinux/demo/install-crossover-21.0.0.bin
chmod 755 install-crossover-21.0.0.bin
./install-crossover-21.0.0.bin
Activate critical 32 bit libraries so that 32 bit games run on 64 bit linux
sudo dpkg –add-architecture i386
sudo apt install nvidia-driver-libs:i386
In Crossover install steam
In Steam install rocksmith and Rocksmith 2014
Run cxdiag – works
Run cxdiag64 – works
Manual Fixes for 32 bit libraries so audio can work perfectly and other things like odbc etc
./cxfix missinglibgsm
./cxfix missinglibasound
./cxfix missinglibpcap
./cxfix missinglibosmesa8
./cxfix missinglibopencl
./cxfix missinglibodbc
./cxfix missinglibcapi20
./cxfix missinggstreamer1libav
Manual fix
apt-get install gstreamer1.0-plugins-bad:i386
Change audio to alsa
Winetricks – wget https://raw.githubusercontent.com/Winetricks/winetricks/master/src/winetricks
chmod +x winetricks
./winetricks
set audio to alsa
Run crossover regedit
Export CU/software/wine/drivers
Run bottle command regedit
Import registry file to bottle

Change driver to alsa
Rocksmith Config
Now configure your steam bottle to use alsa audio drivers for Rocksmith to work.
~/cxoffice/bin/wine –bottle “Steam” winecfg
Configure Rocksmith INI
Win32UltraLowLatencyMode=0
Enjoy playing your guitar on linux with rocksmith!
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